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Purified anti-mouse CD182 (CXCR2) [SA044G4]; Isotype: Rat IgG2a, κ; Reactivity: Mouse; Apps: FC; Size: 100 μg
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Ultra-LEAF Purified anti-mouse CD182 (CXCR2) [SA045E1]; Isotype: Rat IgG2a, κ; Reactivity: Mouse; Apps: FC; Size: 100 μg
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CXCR2 Mouse anti-Human Monoclonal (Unconjugated) Antibody, (50 µg)
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Reported isoforms represent identical protein: NP_001548.1, NP_001161770.1. The immunizing peptide represent part of an extracellular domain.
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Alexa Fluor 647 anti-mouse CD182 (CXCR2) [SA045E1]; Isotype: Rat IgG2a, κ; Reactivity: Mouse; Apps: FC; Size: 25 μg
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Image Search Results
Journal: Mucosal Immunology
Article Title: Butyrate regulates neutrophil homeostasis and impairs early antimicrobial activity in the lung
doi: 10.1016/j.mucimm.2023.05.005
Figure Lengend Snippet: Effect of butyrate on acute Pseudomonas aeruginosa immunity. (A) Quantification of the total number of cells in BALF 18 hours post-inoculation with 1 × 10 6 CFU of P. aeruginosa strain PAO1 in Ctrl and But mice. (B) Frequency and quantification of cell types in cytospins of BALF from control and butyrate-treated mice 18 hours after PAO1 infection; (C) Quantification of P. aeruginosa CFU in BALF 18 hours post-challenge. (D, E) Cytokine and chemokine production in BALF 18 hours following infection. (F) MFI of CXCR2 on lung AM and IM from Ctrl and But mice. (G) MFI of CXCR2 on Ctrl and But lung macrophages US or stimulated for 2 hours with LPS in vitro . (H) transmigration of Ctrl and But neutrophils toward a CXCL2 or CCL2 chemokine gradient after 3 hours. Results are a mean of two independent experiments. Values are expressed as mean ± standard error of mean; n = 6–14. Statistical significance was determined with one-way analysis of variance in (A–E, G, H) and Student’s t test (unpaired, two-tailed) in (F). * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001. AM = alveolar macrophages; BALF = bronchoalveolar lavage fluid; But = butyrate-treated; CFU = colony-forming units; Ctrl = control; IFN = interferon; IL = interleukin; IM = interstitial macrophages; LPS = lipopolysaccharide; MFI = mean fluorescence intensity; PAO1 = P. aeruginosa strain 1; TNF = tumor necrosis factor; US = unstimulated.
Article Snippet: To differentiate progenitor and neutrophil populations in the BM, cells were stained with antibodies to lineage cocktail, CD115 (BD Biosciences, clone 2B8), SiglecF (BD Biosciences, clone 2B8), CD117 BUV117 (BD Biosciences, clone 2B8), Sca1 APCFire (Biolegend, clone D7), FcgR biot (Miltenyi, clone 93), CD34 BV421 (Biolegend, clone SA376A4), CD11b BV510 (BD Biosciences, clone M1/70), Ly6G Pecy5 (eBioscience, clone 1A8-Ly6g),
Techniques: Control, Infection, In Vitro, Transmigration Assay, Two Tailed Test, Fluorescence